To further validate whether LHX2 influenced the cell proliferation of breast cancer in vivo, SKBR-3 and BT-549 cells were stably transfected with sh-LHX2 and a control vector, and they were inoculated into BALB/c nude mice subcutaneously in the left and right axilla, respectively. The gene discussed is LHX2; the disease is breast carcinoma.