We also performed a TUNEL assay on tumor tissues, and compared with the si-NC group, the TUNEL-positive cells of si-LHX2 were significantly increased (Figure 9E), indicating that LHX2 inhibited apoptosis in breast cancer, which was consistent with the results of the Western blotting assay; thus, the results complemented each other. This evidence concerns the gene LHX2 and breast cancer.