In a recent in vitro model, Constance et al. reported that 3D alginate HCT116 cells in multicellular TME cultures consisting of fibroblasts and T lymphocytes can be used to investigate the impact of TNF-β, Sirt1-ASO and/or RES on colorectal cancer proliferation and invasion with a focus on cancer stem cells. The gene discussed is LTA; the disease is colorectal cancer.