ICC staining of α‐SMA and vimentin, scratch migration assay, and 5–ethynyl–2'–deoxyuridine (EdU) assay also demonstrated enhanced fibroblast migration, proliferation, and trans‐differentiation into myofibroblasts after hTGF‐β1 or Ang II stimulation (Figure 4F–I), indicating the successful construction of in vitro fibrotic models. Here, ACTA1 is linked to intrahepatic cholangiocarcinoma.