In addition, we found in CT26 CDX that the sensitized anti‐tumor effectiveness by combining ANO1 knockdown and anti‐PD‐1 antibody was alleviated by TGF‐β treatment (Figure S6A,B, Supporting Information), while the increased CD8 infiltration and decreased α‐SMA distribution in CDX tissues induced by ANO1 knockdown were also reversed by TGF‐β treatment (Figure S6C,D, Supporting Information). Here, ANO1 is linked to neoplasm.