By further analyzing cells’ spatial traits in CT26 CDX tissue, reduced tumor/stroma ratio of CAF distribution and increased tumor/stroma ratio of CD8+ T cell distribution were observed after ANO1 knockdown (Figure 8D), while similar contexts were found for the density and area of CAFs and CD8+ T cells (Figure S5A,B, Supporting Information), indicating that ANO1 knockdown prominently induced a retreat of CAFs from tumor to stroma and an accumulation of CD8+ T cells from stroma to tumor. The gene discussed is DDX53; the disease is neoplasm.