The PIK3CA hotspots p.E545K and p.H1047R are the two most commonly reported mutated PIK3CA sites in endometrial cancer.[10] Thus, Pik3ca E545K cDNA was overexpressed by a retrovirus, together with Trp53 and Pten sgRNAs and Myc overexpression (TPMCa). The gene discussed is PTEN; the disease is endometrial cancer.