As shown in Fig. S2A-D, the results of qRT-PCR and western blotting confirmed that transfection of shM16-1 and shM16-2 significantly decreased METTL16 mRNA and protein expression (Fig. S2A, B), while transfection of pHBLV-METTL16 increased METTL16 mRNA and protein expression in CRC cells (Fig. S2C, D). The gene discussed is METTL16; the disease is colorectal carcinoma.