Also, as NCM460 cells, the less tumorigenic Caco-2 CRC cell line [39, 40] only experienced a mild impairment in cell viability after 72 h post-transfection of siDIS3L2 (DIS3L2 KD), while HT-29 cells, known to show low oncogenicity [41, 42], did not respond to any of the silencing approaches (Fig. 5D–E). The gene discussed is DIS3L2; the disease is colorectal carcinoma.