In CRC patients, EV-derived MARCKSL1 (r = 0.249, P = 0.028), CA125 (r = 0.391, P = 0.002) and lymphocyte (r =  − 0.259, P = 0.025) levels were significantly correlated with distant metastasis. This evidence concerns the gene MARCKSL1 and colorectal carcinoma.