To further investigate the ability of C47 and C61 to induce translational readthrough and determine if they could exert a readthrough activity on another tumour suppressor gene, C47 and C61 were tested in H1299 cells stably transfected with the three nonsense mutant PTEN constructs (R130X, R233X and R335X) with C-terminal deletion following the stop codon and a C-terminal FLAG tag. Here, PTEN is linked to neoplasm.