Previously, Arabidopsis and barley ectopically expressing a double-stranded RNA (dsRNA) targeting three fungal CYP51 genes significantly enhanced plant resistance to Fusarium graminearum species by disrupting fungal membrane integrity, subsequently, spraying detached barley leaves with a 791-nt long CYP3-dsRNAs that contains complementary sequences to CYP51family members prior to fungal infection could effectively inhibit disease and yield much smaller lesions [14,29]. This evidence concerns the gene PPIF and fungal infectious disease.