CD4 and AIDS: Here, we aimed to determine (i) if protection by heat-inactivated ZNF2oe cells can prevent relapse from occurring after challenge, (ii) if ZNF2oe vaccination in hosts with preexisting asymptomatic cryptococcal colonization will have any adverse effects, and (iii) if vaccination with ZNF2oe cells could provide protection to hosts when their CD4+ T cells are depleted (to mimic the immune status of AIDS patients).