Using the ssGSEA and Spearman's rank correlation test of genes, enrichment fraction results showed that the downstream receptor genes of SCFAs perform a clear correlation with the AMPK/NF-κB/NLRP3 pathway, which indirectly demonstrates the possible microregulation of the AMPK/NF-κB/NLRP3 pathway by SCFAs during SAP-ALI (Fig. 6C). The gene discussed is NFKB1; the disease is acute respiratory distress syndrome.