To determine whether any of the EV cargo changes could translate to potential biomarkers of TSC, we isolated EVs from plasma samples from patients with a TSC2 mutation and age and sex‐matched unaffected healthy donors (N = 9 patients/group; patient information tabulated in Table S2) using an established size exclusion chromatography‐based approach (Welton et al., 2015). Here, TSC2 is linked to tuberous sclerosis.