Therefore, this study was designed to investigate in vitro antioxidant potentials, enzymes inhibitory activities (i.e., α-glucosidase, α-amylase, AChE and BChE inhibitions) and in silico docking of the HPLC-identified lead compounds of polyphenolic-rich extract of H. cannabinus (PEHc) against some protein targets that are crucial in T2DM and neurodegenerative disease (AD). The gene discussed is BCHE; the disease is Alzheimer disease.