TP53 and cancer: We specifically selected representatives of zeta, IMP1, Koc, Survivin, c-MYC, RalA, and p53 gene as marker for research, each gene may have different function in the tumorgenesis, among them, the p53 cancer suppressor gene was a very hot gene studied in the last century, we aimed to provide evidence to prove that HELC treatment induces tumor responses in cancer patients to produce iTAA.