To determine if blocking the N-MYC–WDR5 interaction impacts N-MYC chromatin binding, we engineered two neuroblastoma cell lines that lack N-MYC expression, SHEP and SK-N-AS, so that they express an inducible version of wild-type (WT) or WBM N-MYC using the Tet-on system. This evidence concerns the gene MYCN and neuroblastoma.