MAD2L1BP and female infertility: To further corroborate the causal relationship between MAD2L1BP variants and female infertility resulting from oocyte MI arrest, we next interrogated the phenotypic outcome by overexpression of wild-type (WT) or mutant Mad2l1bp (hereafter all ‘Mut’ represents ‘p.R285*’) via cRNA injection into PMSG-primed mouse oocytes at the GV stage (Figure 3A).