Using RT-qPCR, we confirmed that FOS, DUSP1, CXCL8, and NFKBIA were significantly up-regulated in HF (n=20) compared with non-HF (n=40) group in validation dataset, whereas CEBPD, BCL2A1, and SAMSN1 were not (Figure 6E–K). This evidence concerns the gene NFKBIA and hydrops fetalis.