In this study, Abrine and anti-PD-1 antibody were used to treat Hepa1-6 xenograft mice, results showed that both inhibited the expression of CD47 and PD-L1 in tumor tissues of mice, increased the levels of CD4+ and CD8+ T cells, decreased the level of Foxp3+ Treg cells. This evidence concerns the gene CD4 and neoplasm.