In this study, we evaluated the effect of Abrine on anti-HCC and immune response and determined that Abrine as an IDO1 inhibitor could inhibit IFN-γ, PBMCs, or IDO1-induced IDO1-JAK1-STAT1 signaling pathway, enhanced the phagocytosis of macrophages through inhibiting CD47 expression, and decreased the PD-L1 expression in HCC cells. This evidence concerns the gene IDO1 and hepatocellular carcinoma.