However, as the total number of Ki67-positive GBM cells in the brain was not significantly altered by PODXL knockout (as determined by quantification of either 2 or 5 consecutive 50 μm sections per mouse) (Supplementary Figure S4), we were able to determine how PODXL expression influenced the proportion of GBM cells that had migrated from the ipsilateral to the contralateral (left) hemisphere. This evidence concerns the gene MKI67 and glioblastoma.