Here, we investigated the potential of ExoView technology for direct capture and subsequent fluorescent labeling of TF-expressing EVs derived from the human pancreatic cell line BxPC-3, in platelet-free plasma (PFP) containing either TF− EV or TF+ EV, in PFP of trauma patients and platelet-poor platelet (PPP) of ovarian cancer patients. Here, TF is linked to ovarian cancer.