Moreover, the SLC9A3R2+ En subpopulation, whose abundance was significantly reduced in HCC (Figure 4C), was not only significantly enriched in cell adhesion molecule pathways, but was also at an early stage of subpopulation cell differentiation, suggesting that it may gradually differentiate into other endothelial cell stages with the progression of HCC, in turn promoting tumor proliferation and migration (Figure 4H). The gene discussed is NHERF2; the disease is hepatocellular carcinoma.