As simulations predicted that RelA was a critical NF-κB subunit in determining the TME response of DLBCL cells, and previous studies have implicated both cRel and RelB (44, 57), we sought to establish a flow cytometry-based approach that would enable us to characterize the composition of NF-κB proteins in DLBCL with single cell resolution. This evidence concerns the gene REL and diffuse large B-cell lymphoma.