Namely, decreased expression of 4 m5C “writes” (NOP2, NSUN5, NUSN6, DNMT3A) and 2 “erasers” (TET1 and TET3) was closely related to better overall survival, while overexpression of 4 “writes” (NSUN3 and NSUN4) and all ”readers” exhibited a better prognosis in COAD. Here, NSUN4 is linked to colon adenocarcinoma.