Nine m5Cregulators were obtained as prognostic signatures of COAD, and the coefficients of these signatures were utilized to calculate the risk score Riskscore≈NOP2×0.017−NSUN3×0.012+NSUN5\break×0.258+NSUN6×0.641−NSUN7×0.094−TRDMT1\break×0.719+ALKBH1×0.171−ALYREF×0.056−YBX1\break×0.176. Here, NSUN5 is linked to colon adenocarcinoma.