Because there are no previous reports of the cuprizone model applied to X-ALD mice, we first investigated whether Abcd1 KO mice fed a cuprizone diet for 5 weeks, followed by standard chow for up to 7 weeks, develop the expected pattern of demyelination and remyelination in the corpus callosum. This evidence concerns the gene ABCD1 and X-linked adrenoleukodystrophy.