Moreover, it was indicated that in the HCC recurrence model, the cancer stemness pathway (Fig. 9C), the pre-metastasis microenvironment factor (Fig. 9D) and the EMT program (Fig. 9E) in vivo were activated at the mRNA level after adoptive transfer of Th17 cells, and the same trend was reflected at the protein level (Fig. 9F, G), while the function of FOXO1 in negatively regulating Th17 cells and stabilizing the liver microenvironment were confirmed in HCC recurrence models. The gene discussed is FOXO1; the disease is hepatocellular carcinoma.