As shown in Fig. 7C, the colonization rate of GFP+ Hepa1-6 cells in the liver increased significantly after IRI, while the colonization rate was decreased after the upregulation of FOXO1, suggesting that the upregulation of FOXO1 can attenuate the colonization of residual HCC cells in the liver and reduce the susceptibility of the hepatic microenvironment to recurrence. This evidence concerns the gene FOXO1 and hepatocellular carcinoma.