The current study found that compared with negative control cells, the intracellular iron levels and ferroptosis's marker proteins GPX4, SLC7A11, GCLC and NRF2 expression levels significantly declined, ACSL4, LPCAT3 increased, and the microstructure of mitochondria changed in the CM‐treated NP cells, which indicated the involvement of ferroptosis in IDD. Here, LPCAT3 is linked to intervertebral disk degenerative disorder.