The current study found that compared with negative control cells, the intracellular iron levels and ferroptosis's marker proteins GPX4, SLC7A11, GCLC and NRF2 expression levels significantly declined, ACSL4, LPCAT3 increased, and the microstructure of mitochondria changed in the CM‐treated NP cells, which indicated the involvement of ferroptosis in IDD. The gene discussed is ACSL4; the disease is intervertebral disk degenerative disorder.