CALR and neoplasm: There was almost no CRT signal in the PBS, ELNV, and LNV + Laser groups, whereas the ENV + Laser or ELNV + Laser groups dramatically promoted CRT expression on the surface of the tumor cells (Supplementary Fig. 26a), indicating that enzyme-sensitive nanovesicles, whether loaded with LY or not, can both induce cell apoptosis and CRT exposure on the surface of 4T1 cells.