In addition, IF staining and flow cytometry results of PD-L1 expression demonstrated that the ELJNV + Laser group could also block the overexpression of PD-L1 caused by IFN-γ in situ by efficiently releasing JQ1, overcoming the inducible immune resistance, and relieving the immunosuppressive tumor microenvironment in pancreatic cancer (Fig. 6p, r, and Supplementary Fig. 48h). This evidence concerns the gene CD274 and familial pancreatic carcinoma.