In our previous study, we also found that mir-21 could inhibit the Rho-ROCK pathway by decreasing the ROCK1 expression during the process of intestinal barrier protection.18 It is also reported that Rho-ROCK might be an important molecular signal during the regulation of intestinal inflammation, permeability, TJ, and intestinal barrier.22 Moreover, we verified the role of matrine and miR-155 in the DSS-induced mice colitis model and clinical samples of stercoral obstruction patients. This evidence concerns the gene RHO and colitis.