Then the GO term and KEGG pathway enrichment analysis were conducted, and seven immunoregulation-related piRNAs were selected; among them, piR-hsa-27620 and piR-hsa-27124 were not only significantly upregulated in patients with rheumatoid arthritis but also may be involved in immune regulation, which has potential research value as biological markers of RA. The gene discussed is PIR; the disease is rheumatoid arthritis.