Deleting Gli1 Lineage using cre-inducible diphtheria toxin A (DTA) in one-month-old Gli1-CreERT2;DTAflox/flox mice led to coronal and frontal-premaxilla suture fusion after 1-month induction and all craniofacial sutures fusion after 2-month induction with skull growth arrest and osteoporosis. This evidence concerns the gene GLI1 and osteoporosis.