The efficiency of PLK4 knockdown were verified by WB and qRT-PCR, functional validation in vitro including clone formation and the CCK-8 assays were applied to detect cell proliferation abilities, it turned out that PLK4 deficiency considerably restricted the proliferation of CRC cells, however, there was no significant difference observed in the proliferation of the wild-type and control group cells (Figure 1A and Figure S1A&B). The gene discussed is PLK4; the disease is colorectal carcinoma.