Consistent with this, immunofluorescence staining for PECAM-1, NG2, and NF in CC tissue revealed that shCon MCP-EVs significantly improved endothelial cell (Figure 5D and 5F), pericyte (Figure 5D and 5G), and nerve (Figure 5E and 5H) contents in CNI-induced ED mice, thereby ameliorating erectile dysfunction. Here, NFASC is linked to erectile dysfunction.