To evaluate whether Hebp1 delivered by MCP-EVs contributes to nerve regeneration in vitro, we inhibited Hebp1 expression in MCPs by infection with lentivirus expressing small interfering RNA (siRNA) against Hebp1 (shHepb1) and then isolated Hebp1-depleted MCP-EVs for MPG neurite sprouting assays and Schwann cell migration assays; scrambled small hairpin RNA (shCon) was used as a control (Figure 4B-4E). This evidence concerns the gene HEBP1 and infection.