In the same study, the RING domain-deleting RNF6 remains the same size as the wild type in the IB assay28, this result is impossible because the RING domain is composed of 44 amino acids and the molecular weight is around 5 kDa, which could be clearly distinguished 9,29.In the present study, we solidified the conclusion that PBX1/RNF6 was downregulated in NSCLC tissues and suppress cell proliferation. This evidence concerns the gene PBX1 and non-small cell lung carcinoma.