Puerarin protects against hyperglycemia-associated inter-endothelial junction disruption, according to Lian et al. (44) It may restore inter-endothelial junction disruption by blocking Nod-like receptor protein 3 (NLRP3) inflammasome activation, decreasing subsequent caspase-1 activation, and activating the release of high mobility group box 1 (HMGB1) by lowering intracellular ROS levels. Here, HMGB1 is linked to Hyperglycemia.