To clarify whether the action of Kampo-hozai was not a phenomenon observed only in NPY-KO, we evaluated the anxiolytic effect of NYT using wild-type zebrafish with normal NPY using the low water stress test (Figures 5A, B) because, in our previous and preliminary experiments, wild zebrafish did not exhibit anxiety-like behavior in the cold stress and the novel tank test (Kawabe et al., 2021). The gene discussed is NPY; the disease is Anxiety.