Immunofluorescence (IF) or immunohistochemistry (IHC) staining of patient liver tissues is commonly used in clinical practice to identify the presence of these antibodies (Kubitz et al., 2015)Anti-BSEP antibody-associated cholestasis severity correlates with the level of anti-BSEP antibodies in the body. The gene discussed is ABCB11; the disease is cholestasis.