Supplementation of a probiotic like protein (AM-ZHY1) with Amuc-100 anchored to surface of Lactobacillus lactis ZHY1 with high fat diet showed a significant decrease in hepatic steatosis, decreased expression of PPARy, SREBP-1c, FAS, ACC1, CD36, FABP6, TNF-α, and IL6 in the liver, increased expression of intestinal tight junction proteins and reduced abundance of Proteobacteria and Fusobacteria compared to high fat diet alone. The gene discussed is FAS; the disease is Hepatic steatosis.