Supplementation of a probiotic like protein (AM-ZHY1) with Amuc-100 anchored to surface of Lactobacillus lactis ZHY1 with high fat diet showed a significant decrease in hepatic steatosis, decreased expression of PPARy, SREBP-1c, FAS, ACC1, CD36, FABP6, TNF-α, and IL6 in the liver, increased expression of intestinal tight junction proteins and reduced abundance of Proteobacteria and Fusobacteria compared to high fat diet alone. This evidence concerns the gene TNF and fatty liver disease.