Administration of EPS derived from LGG promoted microbial homeostasis, activates HIF1α in the intestine which increases expression of antimicrobial peptides and has decreased expression of pro-inflammatory cytokines in the liver while EPS derived from BL23 induced liver inflammation with an increase in ALT, AST levels in the serum, increased expression of TNF-α, IL-6, IL-10 and gut microbial dysbiosis. EPS from both strains reduced hepatic steatosis in zebrafish on high fat diet and increased levels of intestinal acetate and propionate compared with high fat diet. This evidence concerns the gene IL10 and Hepatitis.