CD335 was positively associated with PD-L1 at both the leading edge and infiltrating zone of glioblastomas (P < 0.01) but not in the core; CD335 also positively correlated with CD8+ T cells as well as with CD68/CD163/TREM2 TAMs only at the leading edge (P < 0.01). This evidence concerns the gene CD163 and glioblastoma.