IDH2 and astrocytoma (excluding glioblastoma): To further investigate the long-term impact of low IDH mutation VAF, we identified 18 subclonal IDH-mutant astrocytoma cases (3.9% of total IDH-mutant astrocytomas) in publicly available datasets7,35 with IDH-mutant TCF ≤0.67 and an additional 156 cases with clonal IDH1 mutation.