FASN and alkaline phosphatase measurement: Regarding factors implicated in cell death, Ripk1 and FASN phosphorylation activated necroptosis and were upregulated in AP compared to the control21, 22; fluctuations in Slc3a2, Pcbp2, and SLC38A1 phosphorylation suggested that ferroptosis was obviously induced in AP23-25 (Fig. 5E).