To further investigate the relationships between FINS levels and IAs in insulin-treated type 2 diabetic subjects, we divided 779 subjects with C-INS into four groups according to their direct FINS levels: group 1, ≤ 15μIU/mL; group 2, 15–30μIU/mL; group 3, 30–45μIU/mL; and group 4, > 45μIU/mL. This evidence concerns the gene INS and type 2 diabetes mellitus.