We injected H22 cells into the bilateral subcutaneous groin of C57/BL6J mice (5 mice/group, 4 groups in total) at the age of 4 weeks, followed by intraperitoneal injection of PBS, afatinib, anti-PD1, or their combination to analyze the tumor growth size and weight for 28 days. The gene discussed is PDCD1; the disease is neoplasm.