The IL-4 and TNF-α in the sera of Zera-Gn-and Zera-Np-treated mice after three immunization doses were significantly higher than those in the control group (p < 0.001), with up to 49.69 pg./mL IL-4 and 21.974 pg./mL TNF-α in the serum of the Zera-Gn group mice; these results indicated that significant Th1-and Th2-type cellular immune responses were induced (p < 0.001), with a dynamic balance between the Th1 type involved in protection against the organism and the Th2-type cellular immune response that contributes to the clearance of the infection (37). This evidence concerns the gene TNF and infection.