Through macro-genetic analysis of intestinal flora, our group found that DHJSD may regulate TNF, NF-kappa B, PI3K-Akt, MAPK signaling pathways through key proteins such as CASP8, TNF-α and IL-3 to slow down the apoptosis of NPCs and thus treat intervertebral disc degeneration [6, 48]. This evidence concerns the gene TNF and Intervertebral disk degeneration.