To elucidate the effects of EIF4A3 in GBM, western blotting method for the determination of EIF4A3 protein levels after knockdown of EIF4A3 in U87-MG and T98G cell lines (Fig. 2a) and overexpression of EIF4A3 in A172 and U251-MG cells with low EIF4A3 expression (Figure S1a), obtaining stable EIF4A3 knockout (EIF4A3-KD) and EIF4A3 overexpressing (EIF4A3-OE) cell lines. Here, EIF4A3 is linked to glioblastoma.