Of significance, the expression of coinhibitory receptors (PD1, KLRG1, LAG3, and TIM3) was considerably lower in CD8+ T cells retrieved from B16 Mgst1 KD tumor (Fig. 9B), and there was an increase in cytokine response (IFNγ and TNFα) along with Granzyme B (Fig. 9C). This evidence concerns the gene TNF and neoplasm.