Figure 3F also illustrates the diversity of macrophage transcript changes with infection, with dramatic up-regulation of Ccl9, modest up-regulation of Cd163, modest down-regulation of Lyve1 and Mef2c, and dramatic down-regulation of Cd180 and Hpgd. Interestingly, by immunostaining, CD180 levels showed little change at this time point (22 hr post-infection; Figure 2G and I), suggestive of a long protein half-life, whereas LYVE1 levels showed a large reduction (Figure 2F and I), suggestive of post-transcriptional as well as transcriptional down-regulation. This evidence concerns the gene LYVE1 and infection.