As a top hit, Slit3 is an estrogen-sensitive axonal guidance molecule previously discussed in the context of endometriosis and pelvic pain.26 Other DEGs include a range of genes involved in inflammation and immune response (eg, Ifi211, Ctla2a, and Tlr4), cholinergic receptors (Chrna3 and Chrnb3), the transcription factor Neurog3, and numerous sex-linked genes. Here, TLR4 is linked to endometriosis.