Furthermore, the collective frequency of missense variants that we predicted were pathogenic in these 2 databases suggests a carrier frequency around 1 in 1,250–2,400 and, therefore, a population prevalence of SGCB-deficient LGMD of approximately 0.2–0.6 per million, ignoring the de novo mutation rate for this gene, which is consistent with various disease prevalence estimates in the literature (14, 17). The gene discussed is SGCB; the disease is limb-girdle muscular dystrophy.