This is the first time to our knowledge that a full-length muscular dystrophy gene has been subjected to DMS, suggesting that pooled functional screens are a viable method of large-scale functional assessment of protein-coding genetic variation in other muscular dystrophy genes (SGCA, SGCD, SGCG, etc.), particularly those whose cell surface expression is key to their function. Here, SGCG is linked to muscular dystrophy.